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The cells at low density (2 K/35 mm) plating remained either single or formed 2 6 celled clusters on d2 of plating exhibiting slower growth (Fig. S1A) compared to the medium and high density plated ones (Fig. S1B, C).
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The electroless Ni B plating exhibits a uniform surface and an amorphous structure.
The TiN-coated surfaces (prepared by arc ion plating) exhibited well-networked surface structures with small dimples on their Ti surfaces.
Petri plates exhibiting bacterial growth were selected, and distinct colonies were streaked on a new set of LB agar media and incubated for 24 h at 30 °C.
E. coli harboring pPhaCAB (left side of the plate) had bright fluorescence under UV light (302 nm) in contrast to E. coli containing pBluescript II SK+ vector (right side of the plate) exhibiting a much weaker fluorescence than above.
Any plate exhibiting >20% variability in the standard curve was deemed to have failed and repeated.
Costar Transwell 24-well plates exhibiting 5-µm pore size were used for all motility assays (Cambridge, MA).
After incubation, the control plates exhibiting 30 300 colony forming units were taken as reference.
∼10 worms were selected from each plate exhibiting a faster rate of food consumption than sibling plates.
Candida colonies were counted on plates exhibiting 30 to 300 colonies to determine colony-forming units (CFU /mL.
STS and all 10 genes identified by the reverse transfection screen led to cultures in 6-well plates exhibiting between 40 70% TUNEL and cleaved CASP3 positive cells after 60 hours compared with only 3 4% in the mock transfection cultures.
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