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RNS60 did not impact the number of cells expressing O4 and GalC assessed one day after plating (data not shown).
In all the mammosphere experiments, primary plating data are provided.
We observed colonies of 20 cells or more appearing between 2-3 weeks after plating (data not shown).
In addition, when attempting to derive cell lines from first-passage MPNST orthoxenografts, a rapid overgrowth of murine fibroblasts was observed immediately after plating (data not shown), indicating the presence not only of murine vessels but also mouse stromal fibroblasts.
However, prototrophic transformants could not be selected because of the background growth of strain M4 on MA5 minimal agar plates even when M4 cells grown in MA5 medium supplemented with uracil were washed extensively before plating (data not shown).
In contrast, the non-invasive MCF-7 and ZR-75-1 cells [ 22], which either lack or have very low expression of COX-2, did not form tubular networks on matrigel at 7 hours; channels were undetectable for up to 48 hours after plating (data not shown).
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Leslie, A.G.W. Recent changes to MOSFLM package for processing film and image plate data.
D.L. developed Volta Phase Plate data collection protocols and contributed to model building.
Hot plate data were analyzed by two-way ANOVA with lesion and drug treatment as independent factors.
His concerns are echoed by a government adviser on transparency who warns that police appear to be illegally retaining number plate data for up seven years.
The FOI request also revealed that the police proposed to increase the retention of number plate data from two years to up to seven years.
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