Sentence examples for plating 1 from inspiring English sources

In brief, 2 days after neuron plating, 1 µM cytosine arabinose (Sigma) was added to the neuronal culture medium to inhibit glial growth for the subsequent 2 days, then replaced with the normal culture medium.

After plating 1 × 104 cells on X-gal indicator plates at 44°C we isolated 7 white LacZ- "pop-in" clones (see Additional file 1).

Cell growth analyses was started 6 days after lentiviral infection by plating 1 × 10 mESCs on 0.1% gelatine coated per 6-well plates in triplicates.

Cell growth rates were compared by plating 1 × 10 cells from each cell line in individual wells of six-well dishes (day 0).

Twenty-four hours after plating (1 day in vitro; DIV), the cell cultures described earlier were exposed to the lentiviral vectors, each at a concentration of 10 ng p24/10 cells.

After the direct plating, 1 ml of trypticase soy broth (TSB) with 0.5% NaCl will be added to the transport tube and the selective broth will be incubated overnight at 37°C in air.

Although dual plating is biomechanically proven to be the best stabilization option, it requires extensive soft tissue dissection with a potential high rate of post-operative complications particularly when single midline incision was used for dual plating [1, 2, 3, 4].

Transient transfections were performed in complete medium plating 1,5×105 cells directly with the transfection mix and adding 750 ng PHOX2B expression construct with 250 ng pGL3basic-ALK promoter, or equimolar amounts of deleted reporter constructs, with 3 µl of FugeneHD (Roche).

Cell line population doubling times (PDTs) were determined in 1 1 ratio indirect cocultures by plating 1×10 cells in each well or insert of a six-well dish.

After direct plating, 24 (38.7%) carcasses were positive.

A parade of odd-numbered plates: 1, 7, 5, 9.