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Six platforms were tested and compared.
For identification, different platforms were tested e.g. CE-MALDI-TOF-TOF (Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry), CE-ESI-QTOF (Quadrupole time-of-flight mass spectrometer) [97] and CE-Orbitrap [98].
Five commercially available microRNA microarray platforms were tested: Agilent Technologies (AGL); Ambion Inc. (AMB); Exiqon (EXQ); Invitrogen (IVG) and Toray Industries Inc. (TRY) (Table 1).
In order to develop reliable RNAi reagents, three different platforms were tested: small interfering RNA (siRNA), antisense oligonucleotide (ASO) and short hairpin RNA (shRNA).
Three array platforms were tested.
The three platforms were tested with a set of 20 primary breast tumor samples.
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As a potential solution, the possibility of extracting nucleic acids on-site using automated platforms was tested.
Data produced from both platforms was tested for outlying values.
and the correlation between GC-content and the differences in expression ratios between platforms was tested.
This package should be validated to work with array data from the variety of microarray platforms being tested.
This global analysis of all BAC and oligo Segments was complemented by an individual comparison, where each segment from BAC or oligo platforms was tested against its corresponding oligo or BAC data set.
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