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The 96-well plates were run on an iQue Screener and data evaluated using FlowJo v.10.0.7 software.
The 96-well plates were run on a high-throughput plate sampler iQue Screener (IntelliCyt) or individually analyzed on an Accuri C6 Flow Cytometer (BD Accuri Cytometers).
The test plates were run through a cleaning process to ensure that there was no further contamination apart from that purposefully applied.
Three plates were run for this experiment.
Plates were run on a Stratagene Mx3000P quantitative PCR system.
Three Stem Cell Array plates were run for each undifferentiated sample and three ECM plates were run for each NPC sample, for a total of 12 plates of each type.
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Plates were ran in a solvent system comprised of methanol chloroform:water:ammonium hydroxide (47 60 11.2 2) to separate phosphoinositol products.
The TLC plates were ran twice using a 15:9:6 (v/v/v) mixture of ethanol, butanol and water as the mobile phase.
The TLC-plates were run in a chamber containing (N-hexane, diethylether, acetic acid and H2O (65/15/1/0.25, v/v).
Then inked moist paper was placed on top of the plate and the plate was run through a printing press.
The plate is run through a computer, and the registration holder is sent a letter explaining that the vehicle was being operated in an unsafe (nay, an aggressive) manner.
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