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Plates were returned to the incubator for 8 10 additional days.
As plates were returned to the kitchen, the music -- a party mix of Cuban salsa and Mexican pop -- was turned up, and random pairings of people took the floor to twirl or swoosh to the music.
The plates were returned to the incubator.
Then, Hela cells were washed with PBS and incubated in a nanogel-free medium and treated with an 808-nm laser at 400 mW/cm2 for 15 min and a 680-nm LED lamp at 10 mW/cm2 for 40 min. For cell survival test, the irradiated plates were returned to the incubator, and cell viability was colorimetrically measured 48 h later with MTT assay [23].
Rediscovered by local historians in the 1990s, they remained at the quarries until 2010, when the surviving plates were returned to Mimoyecques, where they are now on display.
Fresh maintenance medium was added and plates were returned to the incubator.
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If any embryos remained, the plate was returned to the incubator and observed for hatched embryos everyday for the following 3 to 10 days depending on the culture temperature.
After ensuring the trypsin covered the surface, the plate was returned to the incubator for 5 min at 37 °C.
Cells were added (60,000 cells/50 μL) to wells (final concentration of 60,000 cells/100 μL), and the plate was returned to the incubator for 1 h.
Twenty microliters of MTS reagent were added to each sample using a multi-channel pipette, and the plate was returned to the cell incubator for 2 hours.
After an initial 4 h period to allow for cell attachment, we added AB (BioSource, Camarillo, CA, USA) directly into culture medium at a final concentration of 10% and the plate was returned to the incubator.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com