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The plates were maintained in the dark at room temperature (21 C).
The plates were maintained in aerobic condition at 32 °C for 24 48 h.
The plates were maintained in the dark at 26°C for germination.
Then, 4 h later, lipopeptides were added for a final concentration of 65.76 µM, and the plates were maintained at 37 °C for 48 h.
The plates were maintained in an incubator at 37 °C in a humidified atmosphere containing 5% CO2 until the predetermined time points for each assay.
After 24 h, 3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium bromide (MTT) was added to each well (0.5 mg/mL; Sigma–Aldrich), and plates were maintained at 3- 4,5-dimethylthiazol-2-yl -2,5-diphenyltetrazolium
In addition, the test results demonstrated that the proposed design relationships were arranged properly such that the cover plates were maintained in the elastic phase, only slight cracks appeared in the column, and plastic hinges were formed in the beams in the vicinity of the column.
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The plates are maintained at different temperatures.
The gap between the plates was maintained at 3 mm, providing channels through which soluble gas pollutants in the upward gas stream were absorbed by the downward scrubbing liquid film on the surfaces.
Clear 24-well plate were maintained in parallel to assess confluence.
HUVEC cells (8×10 cells/well in the filter of a 48-well transwell plate) were maintained in 10% FBS (v/v) for 48 h.
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