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Infected MEFs are plated onto gelatin coated 24-well plates (Experiment 1) or 24-well plates containing mitomycin-treated feeder MEFs (Experiment 2); cellular reprogramming is initiated by the addition of doxycycline (dox).
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Variations might arise owing to differences in the amounts of material loaded on to the plates, experiments being performed on different days and exposure times for autoradiography.
A pin-on-disk plate experiment is reconstructed to assess the efficiency and validity of the algorithms proposed here.
Five solutions for significantly reducing the effects of out-of-plane motion are presented and three of these solutions are applied to the steel plate experiment.
The divided plate experiment shown in Figure 4B was designed to demonstrate this principle.
Namely, as we start the agar plate experiment, we use a well-mixed homogeneous mixture of two strains.
From the protein agar plate experiment (Fig. 1), we found that those strains hydrolyzed both J. curcas kernel protein and casein upon incubation at 50 °C.
The reactions were performed as a plate experiment, employing an XT-block reactor, with seven organometallic reagents (M=MgX, Li, and ZnX) and ten amines.
Lastly, in every multi-plate experiment CT values for ATPS were always checked; if their range was >1.5 cycles, the samples more distant from the overall CT mean were removed from the study.
Supersize the pie plate experiment by filling a large bucket or plastic bin with Oobleck and jumping up and down in it.
The plating experiments were carried out in ambient condition at room temperature.
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CEO of Professional Science Editing for Scientists @ prosciediting.com