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hPECs were plated on plastic dishes, Matrigel, PCL nanofibers, or PCL nanofibrous microwells.
No statistical significant difference in Rac1 activity was observed between cells plated on plastic vs. collagen I-coated plates.
Media was replenished on the second day and aggregates were plated on plastic 10 cm dishes on the fourth day.
For analysis of cell speed, Dictyostelium amoebae (strain NC4A2) were plated on plastic Petri dishes in HL5 growth medium and allowed to attach for 1 h.
As an initial test of this hypothesis we compared Dvl2-Grb2 synergy in cells plated on plastic versus cells plated on collagen or denatured collagen (gelatin).
The cells were then resuspended in Dulbecco's Modified Eagle Medium (DMEM, Gibco™; Invitrogen, Grand Island, NY), plated on plastic tissue culture flasks, and incubated at 37°C with 5% CO2 to produce mesenchymal stromal cells (SCs).
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After 4-day suspension culture of hiPSC clumps in hESC medium, the hiPSC aggregates were cultured in the neural medium for 2 days and were then plated on a plastic surface.
Neurites plated on flat plastic sample dishes were observed for about 10 minutes.
As shown in Figure 3B, knockdown of ACTN4 decreased the adhesiveness of the cells soon after plating on plastic surfaces coated with fibronectin.
After 48 h, cells were kept in suspension for 1 h before plating on culture plastic.
Primary trypsin technique cultures were plated on uncoated plastic, or plastic coated with type I collagen, type IV collagen, or fibronectin.
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