Sentence examples for plated on medium from inspiring English sources

The construct was used to transform M15 E. coli strains (Qiagen, CA, USA) and the bacteria were plated on medium contained ampicillin (100 µg/mL) and kanamycin (25 µg/mL).

All 26 mutant strains could be cultivated in galactose containing medium but stopped growth when plated on medium with glucose as the sole carbon source (data not shown), indicating that each of the corresponding LSU r-proteins is essential for yeast growth.

Approximately 41,000 Ura+ transformants were plated on medium lacking uracil at 18°.

These plasmids integrated into either flanking region of GSU0299 when transconjugants were plated on medium containing kanamycin.

Yeast transformants were plated on medium containing various concentrations of As III) or Sb III) and growth was scored.

To this end, Mav203 yeast were co-transformed with the MUS81 (1 106 -BD 1 106 -BDbralleled Slibraryand plated on medium containing 5-FOA.

One isolated colony was grown in 300 μl YPD medium overnight, plated on YPD medium and incubated at 30°C for 4 5 days.

Double transformants were patched on the same medium and replica plated on selective medium lacking histidine for auxotrophy analysis as described.

Transformants were cultivated for 25 generations in medium without erythromycin and subsequently plated on solidified medium with and without erythromycin.

Aliquots were withdrawn from each generation and plated on LB medium agar and replica plated on LB medium agar containing kanamycin (5 μg ml-1).

Following pregrowth under permissive conditions in liquid medium, 10 of these cells are plated on selective medium containing lactose as the sole carbon source.