Seeds of A. thaliana (Col-0 ecotype) derived from selfed progenies of an Arabidopsis plant were surface-sterilized for 15 min in 10% bleach, washed four times with sterile water, and plated on half-strength Murashige and Skoog [ 24] medium.
Surface-sterilized seeds were stratified for two days and plated on half-strength Murashige and Skoog medium (MS/2; 0.5x Murashige and Skoog salts with 1% sucrose, pH 5.7).
Seeds of Arabidopsis, both wild-type and transgenic lines, were surface sterilized, plated on half-strength Murashige and Skoog agar medium, and incubated in darkness for 2 days at 4 °C.
The cells were plated on YES, half were exposed to 40 J/m2 UV light and recultured in 5 ml YES.
For observation of seedling phenotypes, sterilized seeds were plated on a half-strength Murashige and Skoog (MS) medium with 1% sucrose and 0.6% agar.
The transformation for the in situ generation of overhangs experiments were split in half after recovery in SOC medium (half plated on ampicillin- and the other half on kanamycin-containing plates).
One half goes on one plate, one half on the other, with the brioche stuffing above the meat and that brown skin above the stuffing.
For in vitro growth studies, A. thaliana seeds were stratified and surface sterilized and plated on growth medium (half-strength Murashige and Skoog medium (1/2 MSigmaSigma), 1% sucrose, 3.25 g/l Phytagel agar; Sigma) as described [21].
Seeds used for seedling root-shoot comparison were surface sterilized using bleach and plated on agar medium containing half strength MS basal salts (Sigma, St . Louis USA).
To grow plants on soil and in axenic cultures, seeds were surface-sterilized in 70% ethanol followed by 50% bleach and plated on MS/2 medium that contained half-strength MS salts (pH 5.7, Sigma, St . Louis MO) and 1% (w/v) sucrose.
