100 µL of the suspension was plated on agar medium containing G418 (100 µg/mL).
After 6 weeks (visit 2), the elastomers were removed, placed in transport media, and plated on agar within 2 hours.
An aliquot of 50 μl of cells diluted were plated on agar LB plates containing ampicillin 100 μg/ml and were incubated at 37 °C for 16 h.
Once plated on agar, all pelotons yielded common conidial fungi (Fusarium sp., Trichoderma sp ., and none resulted in isolates that were assignable to the Rhizoctonia complex.
At different time intervals over the incubation period, samples from each tube were removed respectively and serially (tenfold) diluted, and 100 µl of the dilution were plated on agar plates.
Intestines of larvae were immediately dissected and plated on agar plates containing a bacterial minimal growth medium supplemented either with glucose or sugarcane biomass in the form of bagasse or harvest trash (HT), as sole carbon source.
Novel acetate specialists, in particular, form smaller colonies when plated on agar plates [12], [17], [19].
Following overnight incubation with the mutagen, planktonic cells were plated on agar and fluorescent colonies enumerated.
After overnight growth in liquid medium at 37°C, serial dilutions were plated on agar plates to obtain individual colonies.
Blood and brain were collected and bacterial loads were determined by CFU, serial dilutions plated on agar.
Samples were removed, diluted in 0.9% NaCl, and plated on agar plates to determine the number of CFU recovered from the lysed cell monolayers.
