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Finally, co-transformed cells were re-streaked onto plated medium lacking histidine with various concentrations of 3-amino-1,2,4-triazo 3-amino-1,2,4-triazo 3-amino-1,2,4-triazo 3-amino-1,2,4-triazo
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Briefly, fibroblasts were separated by Percoll density gradient and transferred to plating medium and maintained in culture for up to 96 hours.
LBM cells or VSCs were grown in plating medium for 1 day.
Cells were resuspended in plating medium, counted and plated onto glass coverslips at 1.25 × 105 per coverslip.
Organoids were pelleted at 1,100 rpm, washed four times with digestion buffer and then plated into standard tissue culture plates in plating medium as described previously [ 31].
Two days after plating medium was replaced with fresh medium with or without 5 × 10-9 g/ml recombinant transforming growth factor beta 1 (R&D systems).
Based on the determined cell density, the cell suspension was diluted in plating medium to provide a final cell concentration of 2 × 10 viable cells/mL.
The cells were centrifuged, the medium was replaced with plating medium, and the hepatocytes were transferred to collagen-precoated 24-well plates (Becton Dickinson Labware, Bedford, MA) at a cell density of 1.8 × 10 viable cells/well in 0.5 ml of plating medium and then incubated for 24 h.
Approximately 1×109 cells were plated to medium lacking histidine to select for translocations, and dilutions plated to YPD medium to determine viability.
HCT-116 and MDA-MB231 cells were plated to medium plates at 3×105 cells confluence and treated with combination of thiostrepton and bortezomib for 24 hrs.
Cells were plated in medium without serum.
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