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Figure 2e shows the Cu redistribution layer (RDL) on both the FS and BS of the wafer, which was plated in the same Cu-plating step.
Now, however, the chef is king and the food is totally plated in the kitchen.
BM-MSCs were then plated in the bottom compartment of the Transwell at a density of 2.5×105 cells/well with indirect contaction with H9C2 cells.
This extra lethality was observed when cells were plated in the high-ionic-strength minimal medium normally used for bacterial growth, but was greatly reduced or eliminated by plating on low-ionic-strength medium such as nutrient broth.
One dish, plated in the geometric Gustu style, was a pale log of seared palmito next to a circle of almond sauce with green polka dots of huacataya oil.
Detergent treated cysts of the above cell lines, except acra-acga-, which does not form cysts, were plated in the presence and absence of sorbitol as described for spores.
Cells containing the DAPRS/tRNACUA pair and cat(112TAG) (encoding a chloramphenicol-resistance gene containing an amber stop codon (TAG) at codon 112) were plated in the presence or absence of 6 on the indicated concentrations of chloramphenicol.
To perform the assay, L929 cells were plated in the 96-well plates for 24 h.
The transfected cells were then plated in the culture medium (MEM, 10% FBS, 1X antibiotics) on six-well culture plates.
HEK293 cells (1.5 × 106) transfected with pCMV1-Flag-CCL2 or pCMV1-Flag were plated in the lower chamber.
BMDMs (1.5 × 105) were plated in the upper chamber of the transwell chambers with an 8 µm polycarbonate filter (Millipore, Darmstadt, Germany) in DMEM with 10% FBS.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com