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Isolated cells were plated down as a full-depth chondrocyte population (total cell mass from surface, mid and deep zones) or subjected to a fibronectin adhesion assay as described [33].
Neurospheres from the three lines were dissociated as single cells and plated down on glass coverslips for 2 h prior to fixation and staining with Ki67 (Figure 2A C).
Nonadherent EPCs were plated down on coverglasses coated with poly- l-lysine solution (0.01% vol/wt in H2O).
Recovered mesenchyme was plated down in Hams-F12 (Invitrogen) supplemented with 20% FCS (Invitrogen) and passaged with 0.2% trypsin for up to four passages.
At 2 h after plating, the cells were harvested and assessed for apoA-I and ERK/phospho-ERK expression (secreted apoA-I protein levels could not be reliably quantified by Western blotting in the medium taken from freshly isolated hepatocytes that were plated down for only 2 h; results not shown).
Human dermal microvascular endothelial cells were plated down into six-well plates and grown to near confluence before serum depletion (EGM+1%FCS) overnight.
Similar(6)
Hepatocytes that were isolated from mice receiving myriocin were analysed in experiments 2 h after plating down.
When the voltage tries to pull the plate down, the plate tilts and the bottom of the L inches forward.
So angry he could barely speak, he banged cutlery and plates down on our table.
If the iron is left plate down, the legs automatically raise it above the surface.
He put his plate down, leaned his head back and elbowed her, convulsing.
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