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Phlda2 was highly expressed in the prehypertrophic/hypertrophic growth plate zone (Zone II) (Figure 5A).
The region nearest to the end of the bone is the outermost growth plate zone or resting zone.
Specifically, we compared the NES scores assigned to each cytogenetic locus that was correlated with a particular time point or growth plate zone comparison.
The resulting genes from this intersection were further intersected with genes differentially expressed between two zones of the microdissected growth plate: zone I and II.
In agreement with these data, no significant differences were seen in the length of any growth plate zone in three independent litters (Figure 4B).
Results were quantified by counting worms that reached the attractant spot (zone A), the negative control spot (zone C), or the remainder of the plate (zone B), as shown in Fig. 1A.
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Open image in new window Fig. 1 Growth plate zones, ×10.
Hdac4−/−/Mmp13−/− double knockout mice are significantly heavier and larger than Hdac4−/− mice, they survive longer, and they recover the thickness of their growth plate zones.
We next endeavored to eliminate genes that exhibited changes among all growth plate zones from our subsequent studies.
The expression of Phlda2 was also analyzed by real-time PCR using RNA isolated from the 3 growth plate zones.
Our next objective was to identify molecular classes that were enriched in the different growth plate zones by GSEA.
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