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Lunch, lunch plate, water glass, and silverware all crashed to the floor and shattered, rearranging themselves in a terrible yet somehow thrilling new configuration on the tiles.
b Schematic diagram of inclined solar flat plate water distillation system.
This finding prompted us to plate water samples on LB medium with cefotaxime (60 µg/mL).
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The protocol was adapted to a Sirocco 96-well plate (Waters) where Affi-gel hydrazine resin (Bio-Rad) was used.
Plasma samples were analyzed using solid-phase extraction (Oasis HLB, μ-elution plate; Waters, Milford, MA) and LC-MS/MS.
Samples were mixed with an equal volume of THAP matrix and 1 µL was deposited onto a 96-well stainless steel MALDI plate (Waters).
Digested samples were then desalted by Oasis HLB μElution plate (Waters, Milford, MA) and analyzed by liquid chromatography - tandem mass spectrometry (LC/MS/MS).
Cell culture supernatants were extracted with an Oasis HLB Extraction Plate (Waters Corporation, Milford, MA, USA) and analyzed by high-performance liquid chromatography by using Waters 2795 HPLC Waters Corporationn) coupled to a triple quadrupole mass spectrometer (Acquity TQ Detector; Waters Corporation).
Each sample was acidified using 10% FA to quench the digestion activity, desalted and concentrated on an Oasis HLB μElution Plate (Waters) as previously described [ 14] followed by drying the sample completely in a vacuum concentrator.
The ATP synthase localized by in gel activity bands were excised from the CN-PAGE, chopped into small cubes and transferred to the wells of an OASIS® HLB µElution Plate (Waters Corporation, Milford, CT, USA).
Furthermore, real plating water co-treatment reached equilibrium in 6 h and obtained 1.4/0.9 mmol/g for Zn2+/CrO42−, respectively.
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