Sentence examples for plate washing from inspiring English sources

Microtiter plate washing techniques, as described in Stepanovic et al., also leave room for interpretation and could lead to the removal of transient bacterial biofilms, further adding to the variance in quantitative results for each isolate.

After plate washing, ABTS (Sigma) was added, the reaction was stopped with 1% SDS after 15 min, and the absorbance at 420 nm measured using a Mithras LB940 apparatus.

Secondary goat anti-mouse immunoglobulin G (IgG -peroxidase conjugate antIgG -peroxidase dilution; conjugatem, Santibody, CA, USA) was added after plate washing.

Reported concentrations of MMP-12 were not adjusted for this two fold dilution with Buffer A. Plate washing was performed with a Biotek (Winooski, VT) ELx405 96 channel plate washer.

Dose response curves relating the magnitude of the absorption at 405 nm to peptide concentrations used in the assay plate coating step are found in Figure 2. Nunc Maxisorp assay plates were coated with aqueous solutions of peptide 1 at a concentration of 44.3 μM for 2 h at RT. Assay plate washing and blocking steps were performed as described above.

Then the plate washed twice with PBS and dried.

Media was aspirated, and the plate washed with 1x PBS.

Excess D1D2 was discarded and the plate washed 4 times.

Unbound dye was removed and the plate washed four times with 1% (vol/vol) acetic acid.

At least two cycles of 5 minutes with PBS-Tween 0.05% followed by two cycles of 5 minutes with PBS in an automatic micro-plate washing instrument are recommended.

At least two cycles of 5 minutes with PBS-Tween 0.05% followed by two cycles of 5 minutes with PBS in an automatic micro-plate washing instrument are recommended to remove unattached protein.