Sentence examples for plate transformation from inspiring English sources

Initially, the full collection of yeast mutant strains distributed in seventy-five 96-well microtiter plates (Nunclon) were transformed with the plasmid pJS05 following a protocol of microtiter plate transformation [ 84], using SC-Ura as selection medium.

After selection on the appropriate selective plates, transformation frequencies were determined by colony counting and the calculated transformation efficiencies are shown in Tables 1 and 2. Transformants resulting from chromosomal DNA or integration vectors that were supposed to recombine at the amylose degradation amyE locus were checked for correct integration by testing for amylase activity.

We plated transformation mixtures on Trp- medium, allowed Trp+ colonies to grow, resuspended and pooled the colonies from an entire transformation plate, and then plated these cell suspensions on FOA (Table 1, column 5).

Operations that are required for cloning using the MoClo system consist of preparation of plasmid DNA, liquid handling and incubation to perform restriction-ligation, plating of transformation on plates, picking of colonies, and digestion and analysis of plasmid DNA.

To determine an approximate number of the transformants containing the plasmid that were plated, the transformation suspension was also plated on YPDS agar containing G418.

To improve the correspondence estimation and enhance the interaction between the two steps, an annealing scheme is designed to gradually change the cost minimization from local to global and the thin plate spline transformation from rigid to non-rigid during registration.

The most commonly employed method of generating multicopy expression strains in P. pastoris is based on plating the transformation mixture directly on selection plates containing increasing concentrations of antibiotics (e.g., 100 to 2,000 μg/ml of Zeocin™).

The digested material was then transformed into strain yAAD859, whose genotype is MAT a his3 Δ200 leu2 Δ1 ura3 -52 trp1 Δ63 lys2 -128δ (hhf2 - hhf2 )Δ:: HIS3 (for a description of the generation of the (hht2 - hhf2 )Δ:: HIS3 locus, see Duina and Winston 2004) and Ura+ transformants were then selected by plating the transformation reaction on solid medium lacking uracil (SC-URA medium).

The plates are transformation series occurring in the life of an animal.

Without such thermostable genetic marker systems, false positive colonies would grow on plates after transformation (data not shown).

The resulting shape axes were visualized as thin-plate spline transformation grids (constructed using TPSREGR), which exhibit lucid graphic display based on deformation grids [33].