Usually 7 9 days after plating, spheres with a diameter over 50 μm were counted.
Two possible reasons can be used for understanding the phenomena: first, at the initial stage, two or more kinds of nuclei or silver clusters with different shapes (e.g., plates, spheres, hexagonal, tetragonal, or octagonal) co-exist in the reaction system, which may lead to the formation of plate-like particles and/or spherical particles.
Two weeks after plating, spheres with a diameter over 40 µm were counted.
Upon streaking on agar plates, spheres produced normal colonies, which confirmed the preservation of propagation.
Ten days after plating, spheres (tight, spherical, nonadherent masses >40 μm in diameter) per well were counted, and at least 100 spheres per group were measured.
For percent sphere formation, 100 GSCs were plated per well of a 24 well plate and sphere formation (at 7 days) was calculated using the following formula: percent sphere formation =number of spheres/number of cells plated × 100.
Analogically to literature the main transport resistance is identified in the gap between plate and sphere.
Numerical experiments on low-aspect-ratio flat plates and spheres at Reynolds numbers up to 3700 are used to verify the accuracy and physical fidelity of the formulation.
The main parameters, including volume loss of graphite plates and spheres, micro-morphology of the wear surface and weight and particle size of graphite dust generated, were studied in this work.
To verify its accuracy and to demonstrate its applicability, numerical analyses for a square plate, a sphere and a cylinder, a weapon system and a virtual naval ship have been carried out, of which results have been compared with analytic solutions and those obtained by the other existing software.
