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Individual spheres in single wells of a 96-well plate proliferated over time, and FACS analyses of propidium iodine stained neurosphere single-cell suspensions revealed that approximately 2.72% of sphere cells went through S-phase of the cell cycle, confirming their proliferative capacity.
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Results show that HUVECs adhered to the well plate) and proliferated to reach confluence, but they did not form any network during the culture time).
Cells were counted, plated onto 96 well plates and proliferated for 24 hours before being growth arrested and treated for 24 hours.
Bipolar cells were observed shortly after plating and proliferated rapidly when maintained in this medium.
Cells adhered on the plates and proliferated well without significant differences compared to their un-labeled counterparts.
In particular in mid-neurulae, only a region in the most anterior and posterior-third of the neural plate contained proliferating cells.
The different zones of the growth plate (resting, proliferating and hypertrophic) were clearly distinguishable (Fig. 3A1,B1; with higher magnification shown in A2 and B2), although some abnormal areas of hypocellularity were apparent in the growth plates of transgenic mice (Fig. 3B2; red asterisks).
License plate readers have proliferated across the country, from the Hudson River Valley to San Francisco Bay.
The establishment of primary GF cultures is relatively simple because GFs adhere and spread well on culture plates, and proliferate well without requiring specific culture conditions [21].
For BrdU incorporation assessment, we dissociated the neurospheres and subcultured fetal neural stem cells (5×105 cells/well) in 6-well plates, in proliferating media, and then treated the cells with 10 nM siRNAs.
License plate-reading devices have proliferated among American police departments with negligible debate, and remain a largely unregulated practice.
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