Exact(4)
Our code analyzes multiple tracks per plate, filters analyte spots by color, and automatically generates time-resolved figures of merit.
Prior to assay, the plate filters were pre-soaked in 0.1% polythylenimine (Sigma, St . Louis MO) for 1 h at ambient temperature.
These amplicons were cleaned using plate filters PrepEase™ PCR Purification 96-well Plate Kit, USB Corporation.
PCR products were purified using plate filters PrepEase™ PCR Purification 96-well Plate Kit, USB Corporation and sequenced with ABI BigDye® terminator mix (Applied Biosystems, Foster City, CA, USA).
Similar(56)
First, by a gel-based primer extension assay described above, and second, by a 96-well plate filter binding assay.
Isotope-labeled internal standards are integrated into the kit plate filter to facilitate metabolite quantification (see File S1 for additional information).
Isotope-labeled internal standards are included as part of the kit plate filter for metabolite quantification.
Samples were filtrated through 0.45 μm plate filter and loaded to HPLC for sugar determination.
Isotope-labeled internal standards are integrated into a kit plate filter for metabolite quantification.
Enzyme activity assays in 96-well plate format were performed by transferring 100 μL CPH or 1 mg ICB substrates into a 96-well 'reaction' plate (filter-plate MSHVN4510, Millipore, VWR, Herlev, Denmark) where they were washed once with water.
Serum samples were diluted 1 50 in HNT-PBS, transferred to a soaked and aspirated 0.45 μm filter plate, filtered with the use of the vacuum manifold and collected in an empty 96-wells plate.
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