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Our results suggest that RUK plays a regulatory, rather than catalytic, role in phragmoplast microtubule organization during cell plate expansion in cytokinesis.
Mutations in a novel Arabidopsis gene RUNKEL (RUK) result in cytokinesis defects caused by abnormal phragmoplast organization and arrested cell plate expansion.
Neural plate expansion was measured by in situ hybridization for the eye field marker, rax.
This result suggests that disruption of MFs affected the constancy of cell plate expansion.
These results suggest that the contribution of MFs to cell plate expansion increases with the progress of cytokinesis.
In this study, we have quantitatively examined the temporary changes in MF contribution to cell plate expansion.
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In long bones, the rhythm of growth plate cartilage expansion needs to be in tune with the activity of osteoclasts and osteoblasts.
Single cell suspension was obtained by incubation in collagenase/dispase and DNAse (Roche, Switzerland), filtering through a 40 μm filter to remove remaining aggregates (Fisher Scientific), and plated directly onto 96-well tissue culture plates for drug testing or passaged onto a new low-binding plate for expansion.
For BrdU assays, NS cells were plated into 12-well plates with expansion medium and were incubated at 37°C overnight for recovery.
The alternative is to go ahead with the ankylosed tooth as a support [8] or else to utilize the osteosynthesis plates for expansion.
Dow Polyurethanes is going ahead with the detailed engineering phase of the name-plate capacity expansion of its polyols plant in Terneuzen, The Netherlands.
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