Following a 10 min incubation period, bioluminescence was read with an integration time per well of 1.2 s for four sequential plate determinations.
The data in Figure 2 demonstrate that agar plate determinations of MIC99 and MPC fit well with the boundaries of the selection window seen in vivo at the site of infection for cefquinome.
In conclusion, the data in Figure 2 demonstrate that the mutant selection window agar plate determinations fit well with the piglets infected with E. coli ATCC 25922 of cefquinome treatment in vivo.
One tube was plated onto TSA plates for determination of the number of CFU in each sample and another tube was used for DNA extraction.
The samples removed for titering were serially diluted and plated onto -Trp plates for determination of the total number of transformants.
An aliquot (100 μL) of the supernatants is also transferred into a microtiter plate for determination of the concentration of proteins using Bio-Rad protein assay (Bio-Rad).
AtT-20 cells were seeded at a density of 60,000 cells per well in a 96-well plate for determination of changes in cAMP levels in response to various treatment conditions.
Dilutions of the resulting cell lysates were plated on LB agar plates for determination of intracellular bacterial counts.
IPEC-J2 cells were lysed and lysates were plated on to LB agar plates for determination of adherent E. coli counts.
Colonies were replica-plated onto uracil-dropout plates for determination of pog1Δ ::ura4+ or ura4-D18 markers.
