Some optimization techniques have been widely applied for spore fermentation based on the plate counting.
We used plate counting method to estimate soil heterotrophic bacterial population.
The experimental values measured by plate counting assay reached (8.05 ± 0.70) × 109 CFU/mL (n = 3).
Additionally, the culturable bacterial populations resistant to tetracycline or chloramphenicol were enumerated by plate counting.
The results indicated that the spore yield detected by fluorimetry assay were consistent to that by plate counting assay.
In our study, mice were administered orally with E. coli CICC 21530 (O157 H7), and E. coli in fecal shedding was examined by plate counting.
To quantify the SQR-21 cells attached to the roots, the root samples were ground in a small mortar grand and quantified by the dilution plate counting method.
One milliliter YMB medium was added to the sample while inside the microcentrifuge tube, and the CFU were determined by plate counting on YMB.
More importantly, the current study demonstrated the use of DPA fluorimetry assays as an alternative to traditional plate counting for quantifying spore concentration in the optimization procedures.
