Exact(7)
For DT calculation, cells were seeded, at the density of 1000 cell/cm2, in the first well of a six well plate, counted every 48 hours and re-seeded in the next well of the same plate at the same density (1000 cell/cm2) to the end of the treatment.
Plates were incubated at 37 ± 1°C for 48 h and colonies per plate counted.
The following day (time 0h), the media was changed and the first plate counted using an hematocymeter.
Cultures were incubated aerobically at 37°C for 48 h and then colony-forming units on each plate counted and corrected to the original tissue weight.
Following the exposure, cells were rinsed with PBS, trypsin treated, removed from the plate, counted, appropriately diluted, and plated on triplicate plates for colony formation (7 10 days).
The exact bacterial concentration of these standards was obtained by colony forming unit (CFU) plate counted in Columbia agar blood (Microbiol, Uta, Cagliari, Italy.
Similar(53)
Additionally, the culturable bacterial populations resistant to tetracycline or chloramphenicol were enumerated by plate counting.
Some optimization techniques have been widely applied for spore fermentation based on the plate counting.
The experimental values measured by plate counting assay reached (8.05 ± 0.70) × 109 CFU/mL (n = 3).
We used plate counting method to estimate soil heterotrophic bacterial population.
The sample was taken to plate counting every 1 h until the bacterial cultures become turbid.
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