The proposed autocatalytic mechanism can be applied in general to determine the preferred self accommodating martensite plate clusters in any alloy system.
The martensitic microstructures of NiTi shape memory alloys were examined by transmission electron microscopy and found to be self accommodating conglomerates of large primary (or first formed) plates and smaller secondary martensite plate clusters.
The martensitic microstructures of NiTi shape memory alloys were examined by transmission electron microscopy and found to be self accommodating conglomerates of large primary (or first formed) plates and smaller secondary martensite plate clusters.
Plate clusters of the C.b.[Im11] variant were observed after three days at 16°C in the micro-chip, in condition #26 of the PEG/Ion screen (Hampton Research; 10 µM RNA, 12.5 µM 5'-CGAdT-3', 20% PEG 3350, 200 mM Zn acetate) (Figure 6A).
This showed that all known plates cluster into one of the two groups, with no intermediate forms.What is left, then, is the conclusion that Stegosaurus plates really were different from one sex to the other.
Normal thymocytes (5 × 105 cells/well) from 4 8 week old C3H/HeJ mice (The Jackson Laboratory, Bar Harbor, ME) were incubated in microtitre plates (Cluster, Costar, Cambridge, MA) in cell culture medium at 37°C with 5% CO2 for 72 hours.
Single cells (1000 cells per well) were re-plated and cultivated under normoxia at ultralow attachment six-well plates (ultra low cluster plates, Life sciences).
For general quantitative assessments monolayer cells were trypsinized (or subjected to Cell Dissociation Buffer in case of HEK293) to obtain a single-cell suspension before plating on Ultra Low Cluster Plate (costar cat. 3473) at densities of 300.000 400.000 cells per well.
Briefly, hTERT-OA 13A FLSs were plated in a 24 well cluster plate at 95%% confluence.
Briefly, hTERT-OA 13A FLSs (4×10) were plated in six well cluster plates.
