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The new equipment, called a "plate carrier," would protect vital organs and weigh less than 20 pounds.
The wash step was carried out with the magnetic washing plate carrier of the automated wash station.
Plates were subsequently washed three times on the magnetic plate carrier with 100 μl Bio-Plex (Bio-Rad Labufferries) wash buffer per well.
All washing steps were carried out on a Bio-Plex™ Pro II Wash Station (Bio-Rad Laboratories, Veenendaal, The Netherlands) using a magnetic plate carrier or the vacuum carrier.
Buy a plate carrier with M.O.L.L.E webbing.
Vest or Plate Carrier, you can also buy REAL STEEL gear.
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The following day, well contents were recovered either via inverted centrifugation in custom machined plate carriers for 1 minute at 50×g, or pipetting with large bore "genomic" pipette tips (Molecular Bioproducts, cat# 3531).
We would like to thank Ting Yin and Manuel Alvarez for hESC culture support, Raheem Peerani and Aaron Wheeler for helpful insight and discussion, Maxime Poirier for assistance with design and fabrication of the silicon wafers, Franz Schuh for manufacturing the plate carriers for inverted centrifugation, and Martin Pera for critical reading of this manuscript.
Plate carriers can be expensive.
The best plate carriers are OE-Tech, Pantac (if you have the money) Condor, and NC Star.
When it comes time to finish the vests, MOLLE Chest Rigs and Plate Carriers are a good idea.
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