Women in bright saris wade out into the water, filling small plastic flasks to take home.
These cells, which are fed with a sugar solution and grown in plastic flasks, are a long way from a human with the disease.
The cells were cultivated in square plastic flasks and nurtured in a large incubator.
The moisture-adjusted soil samples were placed in 200 cm3 plastic flasks with lids perforated to allow gas diffusion, and connected directly to the CO2 measurement system.
The cultures were maintained in plastic flasks and incubated in CO2/air (5:95, v/v) at 37°C in a humidified incubator.
Human fibroblasts were cultured in plastic flasks in DMEM (Sigma, USA) supplemented with 5%% fetal bovine serum (FBS) (v/v) (HyClone, USA), gentamicin (150 mg/ml) (Farmak, Kiev, Ukraine), and amphotericin B (5 μg/ml, PAA).
Murine bone marrow cells were collected by flushing the bone marrow cavities of 6-week-old wild-type male C57BL/6 mice euthanized by cervical dislocation and then purified by its physical propensity of adherence to plastic flasks [38].
SPEV cells were cultured in plastic flasks in DMEM (Sigma, USA) with 5% FCS (v/v) (HyClone, USA) supplemented with penicillin/streptomycin (PAA, Austria) and amphotericin B (5 μg/ml) (5% CO2, 95% humidity) as reported by [19].
The cells were maintained as monolayers in plastic flasks in DMEM supplemented with 10% fetal bovine serum, 15 mmol L−1 l-glutamine, 100 units mL−1 penicillin, and 100 g mL−1 streptomycin and grown in a humidified incubator with 5% CO2 at 37 °C.
HT29 cells were cultured in plastic flasks (Nunc, Denmark) in RPMI-1640 medium (Sigma, USA) with 10% FCS (v/v) (HyClone, USA) supplemented with 2 mM L-glutamine (Sigma, USA) and 40 mg/ml gentamycin (Sigma, USA) under standard conditions (5% CO2, 95% humidity) [21].
Monocytes were selected by adhesion to plastic flasks for 1 h.
