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ARE-luciferase plasmid vector was obtained from Cayman Chemicals.
In our case, plasmid vector was transfected into cells, but only mRNAs were injected into embryos.
Cells expressing an empty plasmid vector were used as a control.
pCAGGS expression plasmid vector was a kind gift from J. Miyazaki (Osaka University, Osaka, Japan).
The plasmid vector contains a resistance marker for hygromycin B to permit selection for transformed cells.
Restriction enzyme sites for BglII and EcoRI were added and used for directional cloning into the MIGRI plasmid vector.
For DNA sequence analysis, the PCR products were subcloned into a pCR4Blunt-TOPO plasmid vector (Life Technologies).
Isolated PAC clones were completely digested with BamHI and subcloned into plasmid vector.
For sensitivity testing, the target sequence was ligated into a plasmid vector and cloned.
The widely used lentiviral pLKO.1 plasmid vector is very popular in RNAi studies.
We report the construction of a plasmid vector designed for regulated gene expression in Streptococcus pneumoniae.
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