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Blood Plasma was frozen at −70 °C.
The plasma was frozen immediately and stored at −80 °C.
Plasma was frozen at −20°C for determining rtTA antibody titers.
Plasma was frozen at −20°C for 1 7 days before transport to −80°C freezers for longer-term storage.
For T4 measurement, blood was collected from the saphenous vein in the afternoon and plasma was frozen at −80°C.
Trunk blood was collected in heparinized tubes, centrifuged at 4,000 RPM for 10 min and plasma was frozen at −20°C for later analysis.
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Aliquots of plasma were frozen in liquid nitrogen and stored at –80°C until analyses.
Both erythrocytes and plasma were frozen and stored at −80 °C until analysis.
Aliquots of serum and plasma were frozen at −80°C until analyses.
On arrival, the citrate glass tube was centrifuged and 'buffy-coated' and two tubes of plasma were frozen at -80°C.
Aliquots of plasma were frozen at -20°C and used to measure concomitant plasma inhibin B (in all) and AMH (31 boys and 25 girls) concentrations.
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