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Plasma samples were processed by liquid-liquid extraction (LLE).
Six replicates of each QC plasma samples were processed as usual and analyzed along with six replicates of unextracted standard QC samples by applying correction factor to nullify dilution of extracted samples during plasma processing.
The maternal buccal swab or whole blood sample was first screened to identify heterozygosity at tandem SNP sequences; maternal plasma samples were processed for those that that were heterozygous.
Control plasma samples were processed and stored identically to the targets.
Urine and plasma samples were processed and stored at −80°C for later analyses.
All plasma samples were processed as duplicates in a single assay to avoid interassay variability.
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Each plasma sample was processed using a three-step workflow outlined in Figure 1.
For plasma preparation, blood samples were processed within 30 minutes by centrifugation at 2,000 × g for 15 minutes at 4°C to obtain plasma.
Plasma and urine samples were processed by a solid phase extraction procedure.
Plasma and tissue samples were processed at the indicated times post-injection as previously described [3].
All BALF and plasma (mouse and human) samples were processed, tryptic digested, separated, and analyzed using liquid chromatography-mass spectrometry (LC-MS).
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