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The plasma samples were pretreated by protein precipitation with acetonitrile.
Plasma samples were pretreated by liquid liquid extraction with ethyl acetate.
Plasma samples were pretreated by protein precipitation with methanol.
The plasma samples were pretreated with 0.8 ml of 4% phosphoric acid and loaded on to the conditioned SPE cartridge and rinsed with 2 ml of 4% NH4OH.
To minimize nonspecific binding of other proteins during the immunoprecipitation, all plasma samples were pretreated twice with 20 μl of Novex Dynabeads Protein G (Life Technologies, Carlsbad, CA, USA) per milliliter of plasma and incubated for 1 hour at RT before the beads were magnetically removed as described previously, with some modifications [ 20].
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In this work, plasma sample was pretreated with protein precipitation in order to maximize the extraction of PFCs.
Before analysis, plasma and blood cell samples were pretreated using dilution in a formulated saline buffer with 1% blocking polymer according to the supplier's instructions.
The samples were pretreated by a repetitively pulsed high-current ion beam and plasma source "Rainbow-5" and high-frequency (100 kHz) short-pulsed (8 μs) metal plasma immersion ion surface treatment.
All samples were pretreated with puromycin before RNA extraction.
Milk samples were pretreated at 95 °C for 10 minutes to prevent interference from antibodies in the milk.
Samples were pretreated with tetrasodium pyrophosphate (0.01 M Na4P2O7 · 10 H2O) and submerged in an ultrasound bath for two minutes to disaggregate particles prior to measurement37.
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