Exact(39)
The plasma was obtained by centrifugations at 900 × g for 10 min. Plasma samples were frozen and maintained at -20°C before analysis.
First, the CGRP has a very short half life, barely 7 min, hence we made sure that plasma samples were frozen to −70°C within 5 min of drawing the blood [10, 11].
Plasma samples were frozen at −70°C.
Plasma samples were frozen in liquid nitrogen and stored at −80°C.
Plasma samples were frozen, thawed, and analyzed simultaneously by the Cytokine Analysis Facility at the FHCRC for IL-7, IL-15, and in selected cases IL-2 by sandwich ELISA.
The plasma samples were frozen at −20 °C until testing.
Similar(21)
At registration and at every follow-up visit, plasma samples are frozen for further analysis.
After centrifugation at room temperature for 20 minutes (1500 × g), aliquots of serum and EDTA-plasma samples were frozen consecutively and stored at -80°C until analyzed.
Subsequently the plasma sample was frozen in liquid nitrogen and stored at −80 °C until analysis.
Each plasma sample was frozen and thawed only once.
After centrifugation, the plasma sample was frozen at -80°C until further analysis.
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