Based on the basis of 8 replicate measurements, the limit of detection in tenfold diluted plasma samples was found to be 40 pg/ml and the working range was 50-1000 pg/ml.
The linearity between responses (peak areas) and concentrations of S-warfarin in plasma sample was found in the range of 15.4 3080 ng mL−1 (R2 = 0.999).The linear range for a racemic mixture of R, S-warfarin in plasma which has been obtained by RP-C18-HPLC-UV method, was 12.0 2500 ng mL−1 (R2 = 0.998).
If a plasma sample was found to be positive for one autoantibody, the child was retested every 6 months; if a sample was positive for two or three antibodies, the child was retested every 3 months.
In Experiment 2, a large and variable number of plasma cortisol samples were found to be under the limit of detection of the RIA method, at each time point.
For the optimized method, the linearity between responses (peak areas) and concentration of methadone in plasma and saliva samples were found over the range of 3.6 40,000 ng mL−1 (R2 = 0.997) and 3.0 40,000 ng mL−1 (R2 = 0.998), respectively.
Detection of PPP1R3C methylation alone or its combination with EFHD1 methylation in plasma DNA samples was found to show high sensitivity and specificity, and their sensitivities in early-stage CRCs were substantially higher than that of CEA and CA19-9.
However, in the six tumour models where plasma samples were available, we found no correlation between endogenous plasma thymidine concentrations and [18F]FLT uptake (Additional file 1: Figure S4).
A good linear correlation was established between plasma and saliva misonidazole concentration, and salivary sampling was found to be suitable for the estimation of a number of pharmacokinetic parameters.
Of 87 above mentioned plasma samples, 58 were found to be positive for anti-HCV antibodies using 3 assays: 1) Serodia® HCV (Fujirebio, Japan), 2) Monolisa anti-HCV PLUS Assay Version 2 (BioRad, Marne la Coquette, France), 3) a third generation microparticle enzyme immunoassay (MEIA) (AxSYM HCV, version 3.0; Abbott, Wiesbaden, Germany).
Plasma samples were assayed for total GLP-1 immunoreactivity, as previously described,33 using antiserum no.
