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Plasma samples were extracted by liquid-liquid extraction.
The plasma samples were extracted by solid phase extraction (SPE) and analyzed by UPLC-MS/MS for non-esterified oxylipins, acylethanolamides, N-acylglycines, and monoacylglycerols using modifications of previously described protocols [63].
Urine and plasma samples were extracted by solid phase extraction (further details are provided in the online supplementary material).
Briefly, plasma samples were extracted with strong anion-exchange solid-phase extraction columns followed by HPLC separation and atmospheric pressure chemical ionisation mass spectrometry detection in a selected-ion monitoring mode.
Plasma samples were extracted with chloroform:methanol (2 1) using traditional Folch method for lipid extraction.
Plasma samples were extracted on an Oasis HLB (540 mg; Waters Corp., Milford, MA, USA) solid phase extraction (SPE) column according to the method presented by Sandau et al. (2003).
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Plasma samples are extracted with ethanol before analysis.
DNA from plasma samples was extracted using the Qiamp Blood Kit (Qiagen, Hilden, Germany) following the protocol recommended by the manufacturer.
DNA from the plasma samples was extracted with the QIAamp Blood Kit (Qiagen, Germany) using the blood and body fluid protocol, as recommended by the manufacturer.
Plasma samples were obtained by centrifugation at 8,000 rpm for 10 min, and 50 μL of the plasma sample was extracted with 100 μL of acetonitrile containing 0.05 μM of the internal standard (prepared in house).
Total RNA of plasma sample was extracted from 400 μl of stocked plasma using a miRVana PARIS Kit (Ambion, Austin, TX, USA), and eluted into 100 μl of pre-heated (95 °C) Elution Solution according to the manufacturer's instructions.
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