Sentence examples for plasma samples were diluted from inspiring English sources

Plasma samples were diluted in dilution buffer (1% milk powder in 1X PBS 0.1% Tween 20, 0.02% NaAz) 1∶200 for total IgG (IgGt) and 1∶50 for IgG1, IgG2, IgG3 and IgG4 for all recombinant proteins except for AMA1 for which dilutions used were: 1∶2000 (IgGt) and 1∶500 (IgG1, IgG2, IgG3 and IgG4).

Yolk and plasma samples were diluted based on predetermined working dilutions for adult plasma, yolk and nestlings of each sample age group nestling age (Table S1).

Briefly, the thawed plasma samples were diluted 1 100 in PBS SVAA, Uppsala, Sweden) and 100 μL of each diluted sample was added to the pre-coated plates.

Heat inactivated plasma samples were diluted 10 and 100 fold followed by a two fold serial dilution up to 1600 fold.

For IgG measurements, maternal and cord plasma samples were diluted 1 200 for all recombinant proteins except for AMA-1 where a dilution 1 2000 was used.

Acceptable recovery values [23] were obtained when plasma samples were diluted 10-fold with PBS.

To assess protein binding, plasma samples were diluted with acetonitrile and centrifuged at 4000 g.

The plasma samples were diluted 1 10 (v/v) in water and spiked at different concentrations ranging from 0.3 to 5 μM.

Next, we choose the three significant differentially expressed complement proteins (C3, CFI, and C4BPα) for ELISA validation by using individual samples from the bipolar II (n = 20), MDD (n = 30), and HC groups (n = 30), plasma samples were diluted 1 10,000 for C3 analysis, 1 2000 for C4BPα analysis, and 1 1000 for CFI analysis.

Macaque plasma samples were diluted 1∶25 with blocking solution.

Plasma samples were diluted 1∶10 (ANG-1) or 1∶5 (ANG-2).