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Plasma samples were analysed using a validated analytical method.
Plasma samples were analysed by HPLC and UV absorbance detection.
Six blank plasma samples were analysed using HPLC MS/MS after extraction to estimate the specificity of the investigated method.
Human plasma samples were analysed between 0 and 12 h to evaluate the pharmacokinetic parameters of diclofenac.
In addition, plasma samples were analysed by radio-HPLC for the in vivo stability of the peptides.
Heparinized blood samples were collected between 1 and 192 h after treatment and the plasma samples were analysed by high performance liquid chromatography (HPLC) for TCBZ, TCBZ sulphoxide (TCBZ SO), and TCBZ sulphone (TCBZ SO2).
In total, 480 plasma samples were analysed for TNF-α, IL-1β, IL-6 and TGF-β1.
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ALT and AST Activity Measurements-Plasma samples were analysed using an ADVIA 2400 Chemistry System (Siemens Healthcare Diagnostics. Homocysteine Measurements-Hcy concentrations were determined in plasma and urine using the ADVIA Centaur CP Immunoassay System Siemens Healthcare Diagnostics Ltdd).
In February 2012, plasma and dried plasma spot samples were analysed for IP-10 using an in-house IP-10 ELISA.
Venous plasma glucose samples were analysed by the hexokinase method (Boehringer Mannheim Automated Analysis for BM/Hitachi System 717).
Human sodium heparin plasma pharmacokinetic samples were analysed for irinotecan and SN-38 concentrations at Eurofins AvTech Laboratories (Portage, MI, USA) using a validated, sensitive, and specific high performance liquid chromatographic method with fluorescence detection.
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