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Then, plasma samples were allowed to thaw at room temperature.
Prior to extraction, plasma samples were allowed to thaw at room temperature.
Plasma samples were allowed to thaw at room temperature just before use.
Patient plasma samples were allowed to thaw at room temperature before analysis and were run in duplicate.
Plasma samples were allowed to thaw for 50 minutes at room temperature before centrifugation for 5 minutes at 1000 rpm, followed by distribution into Eppendorf tubes in the amounts required for each assay kit.
Plasma samples were allowed to thaw to room temperature and re-spun in a bench-top centrifuge to remove any remaining cell debris as recommended (Page et al, 2006), and cfDNA was isolated as described previously (Page et al, 2011).
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Samples were allowed to liquefy, centrifuged, and the supernatant (seminal plasma) was stored for further analysis.
Serum samples were allowed to clot at room temperature, and then centrifuged and processed as described for plasma.
Stored plasma samples were analysed to allow retrospective reconstruction of what happened to liver enzymes in the majority who did not develop diabetes, and in those who were about to develop diabetes.
Plasma samples were assayed for total GLP-1 immunoreactivity, as previously described,33 using antiserum no.
After centrifugation, plasma samples were kept frozen at −20 °C till further analysis.
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