Sentence examples for plasma samples using the from inspiring English sources
miRNA was extracted from 200 µl of plasma samples using the miRcute miRNA isolation kit (Tiangen Biotech (Beijing) Co., Ltd.; Beijing, China), dissolved in 30 µl of RNAase-free water, and then stored at −80 °C for the subsequent experiments.
Results of the measured concentrations in 97 human plasma samples using the three methods show that the difference between any two methods or among the three methods presents 100% of samples with less than 20% for all the three amino acids and majority of them are under 10%.
Table 1 Examples of protein detection in 19 plasma samples using the PlasmaDeepDive™ kit and SpectroDive™ software with a q value <0.01 Protein Reproducibility of detection in all series, % Series #1 Series #2 Series #3 Totala Number of tech.
Small RNA was enriched from all plasma samples using the mirVana PARIS RNA isolation kit (Ambion, Austin, TX).
Viral RNA was extracted from plasma samples using the COBAS AMPLICOR HCV Specimen Preparation Kit v2.0 (Roche) according to manufacturer's instructions.
Sequences were obtained by nested RT-PCR amplification of RNA extracted from plasma samples using the QIAamp viral extraction kit (QIAGEN GmbH, Hilden, Germany) and further automatic sequencing using the ABI Prism 3100/Genetic Analyzer (Applied Biosystems, CA).
Total RNA was isolated from 60 μl of each plasma sample using the miRNeasy mini kit (Cat no 217004, Qiagen).
Viral RNA was extracted from plasma samples using either the QIAamp Viral RNA Mini Kit (QIAGEN) or the COBAS AMPLICOR HCV Specimen Preparation Kit v2.0 (Roche) according to manufacturer's instructions.
The IP-10 levels were measured in the plasma samples using human IP-10 ELISA Set (R&D Sysytems, USA) as per the manufacturer's instructions [ 33, 36].
Ninety-six proteins known to be involved in cancer were measured in the plasma samples using a Luminex 100 instrument.
RNA was extracted from the plasma samples using Column Viral RNAout (TIANDZ, Beijing, China), and was reverse transcribed using random hexamers to synthesize the first-strand complementary DNA (cDNA).
