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(A) The metabolite-corrected plasma samples.
Plasma samples were analysed using a validated analytical method.
Plasma samples (1 mL) were added to the sample tubes.
Plasma samples were pretreated by protein precipitation with methanol.
The obtained plasma samples were stored at −20 °C.
The plasma samples were thawed at room temperature.
Plasma samples were extracted by liquid-liquid extraction.
c Nucleosomes were also measured on plasma samples.
Plasma samples were also prepared in the corresponding time points.
Plasma samples were analyzed by the LC MS/MS method.
Plasma samples were stored at −80 °C without refreezing.
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