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From these patients, a second ETDA plasma sample was obtained 12 months after the first venipuncture.

The first plasma sample was obtained after peak viremia, and the median plasma viral load was 2,300 copies/ml (interquartile range: 1,400 to 35,500 copies/ml).

In some cases, a paired serum or plasma sample was obtained at the time of surgery or biopsy.

The total amount of lipids in each plasma sample was obtained by using an established summation formula based on serum cholesterol and serum triglyceride concentrations [ 37].

Then, 1 mL aliquots of the plasma were transferred to 1.5 mL tubes and centrifuged at 16,000 × g for another 10 min. After this step, white particles (probably cellular debris) were collected at the bottom of the tube and the supernatant, the two-step centrifugation plasma sample, was obtained and transferred to a fresh tube (about 2 mL).

A series of plasma samples was obtained from two individuals suffering an acute phase response.

Plasma samples were obtained over a 14 h interval.

These plasma samples were obtained for another study, using the same setup (data not shown).

Human plasma samples were obtained from eight healthy individuals according to institutional bioethics approval.

Plasma samples were obtained on admission from SAP patients and at study entry from healthy individuals.

Serum and plasma samples were obtained by centrifuging for 10 min and were stored at −80 °C until analysis.