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Capacitated, acrosome-reacted sperm and zona-free eggs were used in assays designed to define the effects of inhibitors on sperm egg plasma membrane binding or fusion.
Binding of Aβ peptides to the plasma membrane was sufficient to induce trafficking inhibition where peptides with reduced plasma membrane binding and internalization had lesser effect on mitochondrial motility.
These data indicate a role in gamete fusion for one or more zinc metalloproteases of the matrixin and/or adamalysin families that act after plasma membrane binding and before sperm egg membrane fusion.
We also found that Aβ peptide with Icelandic mutation A673T affects axonal trafficking of mitochondria but has very low rates of plasma membrane binding and internalization in neurons, which could explain its relatively low toxicity.
It was shown that Cln/Cdc28 phosphorylates Ste5 on eight CDK sites that flank a plasma membrane binding domain.
Membrane binding and autoradiographic studies on rat brain suggest the existence of polyphenol specific plasma membrane binding sites, possibly underlying the neuroprotective action of polyphenols [21].
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Platelet microparticle formation and exposure of plasma membrane-binding sites for coagulation factor Va were then analyzed by flow cytometry.
The activation of the NOX complex requires proper assembly of the plasma membrane-binding and cytosolic protein components, and the activation is initiated by phosphorylation of the cytosolic complex [ 19, 20].
3) Multiple lines of evidence are presented for a model in which the tail-phosphorylated PTEN (4p-PTEN) more favorably adopts a compact structure, which may feature the negatively-charged tail in a buried state that reduces plasma-membrane binding.
They transduce the signal across the plasma membrane by binding with these ligands that causes certain conformational changes into the seven trans-membrane alpha helices of GPCRs [8, 9].
The PH domain directs AKT translocation from the cytosol to the plasma membrane by binding to the membrane lipids phosphatidylinositide (PtdIns)(3,4 P2 and PtdIns 3,4,5 P3, which are products of phosphatidylinositide-3-kinase (PI3K).
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