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Anatum (Table 2), which is immune to phage O1 when tested by a plaque test (data not shown).
In another human pharmacology study, dapsone was found not to possess antipsoriatic efficacy in the psoriasis plaque test [ 168].
This approach would be similar to the phage typing of bacteria but would differ in that typing by a plaque test would consume much more time than identification by the ELISA test.
Moreover, while the plaque test is strongly affected by different ways of phage exclusion, the ELISA assay is affected only by the presence or absence of a phage receptor on the bacterial surface.
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Bacteriophage presence and titer in the filtrate were assessed by the plaques test using the double-agar layer technique (Adams 1959).
In four of the seven mixed plaques tested, all of the deletion mutants identified contained the Alu I site, whereas for the other three, none of the deletion mutants contained the site.
To recover the 402 bp gene 20 deletion mutant, ten mixed plaques were picked and re-plated and single plaques tested by PCR; at least one pure mutant was identified in seven of these, although at greatly varying frequencies (1/15, 2/15, 3/16, 10/15, 2/17, 2/24, 2/15; 19.8% average).
Immunohistochemical analysis revealed the presence of annexin V in all plaques tested.
The results described here indicate that plaque reduction test is a convenient and more precise method to detect the antibody against the phage-displayed peptide.
Immunogenicity was assessed using a 50% plaque neutralization test.
The plaque reduction test elicited the approximate EC50 of 0.47 μM for curcumin against influenza viruses [ 30].
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