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Controls showed no plaque staining.
The majority of the noncancer serum samples also showed reactivity to BMI-1, but this was generally weaker as determined by intensity of plaque staining.
The lack of cotton wool plaque staining using NT4X-167 further strengthens the possibility that it may prefer binding to Aβ4-40 as compared to Aβ4-42 aggregates.
Similar to the Tg2576 murine model, PS1/APP mice showed extensive plaque staining [ 34, 35], but no significant difference in the retention of [C]PIB.
In solid plaque material removed from the site of the ruptured plaque, staining for TLR4 was localized in infiltrating macrophages and was positive in all solid plaque samples.
Virus was released from cells by multiple rounds of freezing and thawing and titered on permissive BHK cells or BSC40 cells by plaque staining assays.
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Larger and denser Aβ deposits around vessels or ring-like plaques staining for Aβ42 instead of Aβ40 have been reported with some APP mutations compared with SAD [ 33, 34].
In all brain sections from transgenic APP23, Tg2576 and APPswe-PS1dE9 mice incubated with [ 18 F]flutemetamol in vitro, the bound radioactivity co-localized well with fibrillar Aβ plaques stained with ThS and anti-Aβ1-40 antibody (Figure 4).
Thoraco-abdominal aortae were fixed with 4% paraformaldehyde and plaques stained with ORO for en face analysis.
To control for the effect of autofluorescence, plaques stained only with donor fluorophore were subjected to the same analysis; no significant FLIM signal was observed.
These plaques stained with Alcian blue and with PAS at their borders (Fig. S8).
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