Sentence examples for plaque selection from inspiring English sources
The DEN2-PDK-53-based chimeric viruses have recently been characterized by multiple plaque selection and spot/genomic sequencing [23].
The virus was purified through consecutive plaque selection using GFP as a surrogate marker under a fluorescence microscope.
Recombinant BV, abbreviated BV-H6MA-CA, was isolated using the blue plaque selection method as above.
Positive Sf9 cells harboring recombinant BVs were isolated using the blue plaque selection method after beta-galactosidase staining.
Then the amplified recombinant pFastBac HT B-RIP3 constructs were transformed into DH10Bac cells to get the recombinant Bacmid by 'white-blue plaque selection'.
Furthermore, we used antibody recognition and random plaque selection during biopanning of the cDNA libraries to minimize the confounding effects of autoantibodies unrelated to sarcoidosis.
Indeed, initial comparisons of the South African reference and vaccine strains of BTV-6 and BTV-9, suggest that multiple passages in embryonated chicken eggs (60 or 70 passages respectively) and in BHK cells (7 or 11 passages respectively) as well as multiple plaque selections [63], have led to few if any changes in RNA sequence (<0.4% variation in Seg-2, <0.18% variation in Seg-3 ) [65], [65].
However, this method allows for considerable within-plaque selection; despite the tight bottleneck, the ~4 generations of virus growth needed to produce a visible plaque allow for positive selection occurring during this process [ 35].
All phage mutants were isolated from a single plaque on selection S. aureus strains shown next to the corresponding mutants.
His hand-written 1899 property deed hangs unassumingly on the wall next to a plaque announcing the selection of Duarte's as an "American Classic" by the James Beard Foundation.
A small-plaque phenotype was the primary selection criteria to select viral strains for additional serial passage in cells for further attenuation and vaccine development.
