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Virus was assayed by the plaque method.
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Rotaviruses carrying rearranged segment(s) (r-RV) can package up to 10% additional bp without morphological modification [34] nor being defective, since r-RV can be isolated by limit dilution or plaque methods [11], [12], [16], [29], [31].
Intracoronary thermography is a currently used vulnerable plaque detection method.
As regards this study, EVs were quantified by the plaque assay method in confluent monolayers of BGM cells grown in cell culture plates (Bae and Schwab 2008).
The virus was quantified by the plaque assay method of Wentworth and French [77] with MEFs, as reported previously [78].
For each cleaning agent experiment, including water alone, a positive control experiment was performed to ensure that the plaque assay method worked under these conditions.
Recombinant plaques were screened by plaque hybridization method.
Recombinant virus was distinguished from the wild type virus by using the plaque assay method [ 28].
Viruses were harvested from infected MDCK cells and titrated according to the plaque formation method.
Recombinant BV, abbreviated BV-H6MA-CA, was isolated using the blue plaque selection method as above.
Positive Sf9 cells harboring recombinant BVs were isolated using the blue plaque selection method after beta-galactosidase staining.
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