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These plants were subsequently backcrossed to Jin 23B by mixing pollen grains.
The inoculated plants were subsequently kept under a 12/12 (day/night) photoperiod at the same temperature and relative humidity.
The plants were subsequently placed into a tumbler to which 10 third instar N. lugens were introduced.
For the treatment group, the old leaves of the seedlings were removed, and the plants were subsequently transferred to CF air for 7 days to permit the development of new leaves.
Plants were subsequently mock-inoculated or treated with a 50 sporangia per µl suspension of Cala2 or Waco9 H. arabidopsidis isolates, respectively, using a spray gun.
We observed that at concentrations normally used for transient expression assays, AtGV3101 caused a significant decrease in ABA levels and elicited moderate callose deposition, and when plants were subsequently inoculated with P. syringae, we observed reduced SA synthesis and high levels of callose deposition.
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Although an intrinsic mechanism was proposed, cross-pollination with neighboring plants was subsequently put forward as the more likely explanation for the appearance of these wt revertant offspring.
When its pathogen successfully defeats the targeted R gene, plants are subsequently compelled to evolve a much stronger defense mechanism to survive.
By reducing the frequency of encounters with the new pesticide/toxin, this initial step of behavioral adaptation might delay the evolution of physiological resistance if the treated/toxic plants are subsequently used more extensively (see also [ 10]).
We tested the hypotheses that (i) growth will be reduced in the M and E temperature treatments compared with C; (ii) A will be affected much more than R by changes in temperature; and (iii) prior exposure to the warm and cool periods will alter the rates of A or R when plants are subsequently exposed to those conditions compared with plants not previously exposed.
Twenty M3 seeds derived from each unique M2 plant were subsequently planted in rows, and individual plants were tagged and harvested in subsequent generations such that each plant could be tracked back to its unique M2 source.
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CEO of Professional Science Editing for Scientists @ prosciediting.com