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More than 20 T1 plants were screened, and representative lines were chosen for further characterization.
Transgenic plants were screened and characterized as previously described77,78 and T3 homozygous progenies were used in this study.
The generated plants were screened by PCR analysis for the presence of transgene.
Two plants were screened per race and in each plant 5 leaves were inoculated.
After stably transformed by Agrobacterium, target regions of transgenic plants were screened.
OsRap2.6 RNAi plants were screened using PCR with Rap2.6 primers listed in Table 2.
Homozygous transgenic rice plants were screened by using 70 mg/L hygromycin B (InvivoGen, USA).
The NIL-F2 plants were screened with the polymorphic markers ID08.26, RM502, RM447, RM6845, and RM477 to identify the homozygotes and recombinants within the target region.
Homozygous plants were screened for by PCR.
Transgenic plants were screened for herbicide resistance against Basta (300 mg/l).
A total of 33,000 plants were screened in five batches.
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