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In contrast to the up-regulated pattern of differentially expressed RP genes in roots of Pi-deficient plants, expression of all the 81 RP genes was decreased upon Fe deficiency.
In these plants, expression of MRP1 did not increase plant resistance to antimony salts (Sb III)), a classical glutathione-conjugate substrate of MRP1.
After selection of kanamycin-resistant tobacco plants, expression of fusion proteins was proven by immunoblotting.
In leaves and petals of KS110 plants expression of GFP was restricted to the abaxial epidermis.
In marker free plants, expression of ASAL was also confirmed by western blotting and ELISA analysis.
In MeJA- or ACC-treated plants, expression of SlMKK4 was strongly induced by both MeJA and ACC, reaching 3 4 folds of increased at 6 hr after treatment.
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Plant expression of Bacillus thuringiensis (Bt) crystal (Cry) insecticidal proteins have been the primary way to impart insect resistance in GM crops.
In addition, two other Agrobacterium strains were grown: a strain containing a 5' viral vector containing an Arabidopsis SUMO gene and a strain containing a construct for plant expression of recombinase [25].
Both expression constructs were used for transient plant expression of PfGAP50.
Since most of the site-specific recombination systems are from prokaryotic organisms, plant-codon optimization should increase plant expression of recombinases.
This is exemplified by the isolation and transgenic plant expression of the entire Δ8 DHA pathway from a single algal species, Pavlova salina (Petrie et al. 2010b).
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